Journal: Scientific Reports
Article Title: Chronic hypoxia for the adaptation of extracellular vesicle phenotype
doi: 10.1038/s41598-024-73453-1
Figure Lengend Snippet: Extracellular vesicle production and content in different oxygen conditions. Panels A - C show values for HepG2, panels D - F show values for PC3. (A, D) Mean number of particles produced in 72 hours in different oxygen conditions normalised to cell number. (B, E) Mean size of particles produced in 72 hours in different oxygen conditions (C, F) Mean protein content of EVs produced in 72 hours in different oxygen conditions. Normoxia refers to culture under standard tissue culture conditions with atmospheric oxygen. Acute hypoxia refers to culture of cells in 5% O 2 hypoxia for 72 hours. Chronic hypoxia also refers to culture of cells in 5% O 2 with at least two weeks additional culture in 5% O 2 hypoxia prior to experimentation. Error bars in all panels are SEM. Asterisks (*) denote significance determined by one-way ANOVA with Tukey’s HSD test, * = p ≤ 0.05, ** = p ≤ 0.01, *** = p ≤ 0.001.
Article Snippet: Cells were cultured under either normoxic conditions in a standard cell culture incubator (5% CO 2 , O 2 uncontrolled), or in hypoxic conditions using a SCI-tive-N hypoxia workstation (Baker Ruskinn) (5% CO 2 , 5% O 2 , 90% N 2 ).
Techniques: Produced